Reticulon 1 antibody (N-Term)

Catalog No. ABIN243839

The Mouse Monoclonal anti-Reticulon 1 antibody has been validated for WB, IHC, IHC (fro), ICC and FACS. It is suitable to detect Reticulon 1 in samples from Human, Rabbit and Monkey.

Quick Overview for Reticulon 1 antibody (N-Term) (ABIN243839)

Target: Reticulon 1 (RTN1)

Reactivity: Human, Rabbit, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Reticulon 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-Reticulon 1 Antibody

Binding Specificity: N-Term

Specificity: Recognizes an epitope located within the region of aa421-589 of the neuroendocrine specific protein Reticulon-1A (NSP-A), which is also present in the N-terminal part of Reticulon-1B (NSP-B). In normal tissues, this clone reacts with brain Purkinje cells, pancreatic islet cells, cells in the pituitary gland and some (peripheral) nerve fibers. In addition, a few epithelia show positive staining. Species cross-reactivity: Human, rabbit and rhesus monkey.

Purification: Protein G purified

Immunogen: Small cell lung cancer line NCI-H82.

Isotype: IgG1

Application Details

Application Notes: Approved: Flo (1:10 - 1:100), ICC, IHC, IHC-Fr, WB (1:50 - 1:500)

Usage: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunocytochemistry and Western Blot. Flow Cytometry: 1:10-1:100. Immunohistochemistry (frozen sections): 1:10-1:100 using ABC detection. Western Blot: 1:50-1:500. Optimum dilutions to be determined by the researcher. Hybridoma: Derived by fusion of Sp2/0-Ag14 mouse myeloma cells with spleen cells from a Balb/c mouse.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.05 % sodium azide, 40 % glycerol. No stabilizing proteins added.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for Reticulon 1

Target: Reticulon 1 (RTN1)

Alternative Name: RTN1 / Reticulon 1

Background: Name/Gene ID: RTN1
Family: Reticulon

Synonyms: RTN1, Reticulon-1, NSP, Reticulon 1

Gene ID: 6252

UniProt: Q16799